Emergence of AmpC enzyme and antibiotics resistance in gram-negative bacilli.
- Author:
Wen-en LIU
1
;
Yuan JIANG
;
Yin TANG
;
Hong-mei WANG
Author Information
1. Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China. liuwenen@hotmail.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Bacterial Proteins;
analysis;
Child;
Child, Preschool;
Drug Resistance, Microbial;
Drug Resistance, Multiple, Bacterial;
Female;
Gram-Negative Bacteria;
drug effects;
enzymology;
Humans;
Infant;
Male;
Microbial Sensitivity Tests;
Middle Aged;
beta-Lactamases;
analysis
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):134-137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between emergence of AmpC enzyme and drug resistance in the gram-negative bacilli in Xiangya Hospital and to provide information for the treatment of antibiotics.
METHODS:The bacteria were identified; the susceptibility was determined by Microscan microbiological identification system; and the AmpC enzyme was detected by disc diffusion method.
RESULTS:Of the 204 gram-negative strains, the positive rate of AmpC enzyme was 19.61%; AmpC-positive strains resisted to ceftriaxone, piperacillin, ceftazidime, cefotaxime, cefoperazone, aztreonam, piperacillin/tazobactam, cefuroxime, ceftazidime/clavulanic, and cefotaxime/clavulanic. But AmpC-negative strains were susceptible to those antibiotics. There were significant differences, between the two groups (All P < 0.05). Both groups were susceptible to cefepime, imipenem, meropenem, cefoperazone/sulbactam, levofloxacin, and amikacin.
CONCLUSION:The detection of AmpC enzyme is helpful to the screen of drug resistant strains. Most bacilli with AmpC enzyme show the resistance to third-generation cephosporins, aztreonam, and the beta-lactamase inhibitors, but susceptible to imipenem, cefepime, meropenem, cefoperazone/sulbactam, levofloxacin, and amikacin, which provides the information for the antibiotic therapy.
