HSF1 inhibits heat stress-induced apoptosis in Raw264.7 macrophages.
- Author:
Shun-Mei E
1
;
Wei-min XIAO
;
Kang-kai WANG
;
Qiu-peng WANG
;
Mei-dong LIU
;
Ke LIU
;
Xian-zhong XIAO
Author Information
1. Department of Pathophsiology, Xiangya School of Medicine, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Cells, Cultured;
DNA-Binding Proteins;
pharmacology;
Heat Shock Transcription Factors;
Heat-Shock Response;
Macrophages;
cytology;
Mice;
Rats;
Transcription Factors;
pharmacology;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2006;31(2):162-166
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of heat shock factor 1 (HSF1) on heat stress-induced apoptosis in Raw264.7 macrophages.
METHODS:Raw264.7 cells transfected with pcDNA3.1 and pcDNA3.1-HSF1 were exposed to heat stress (42.5 degrees C +/- 0.5 degrees C) for 1 h and recovered at 37 degrees C for 6, 9, 12, and 24 h respectively. Flow cytometry (FCM), Hoechst 33258 staining and DNA ladder assays were performed to assess the apoptosis.
RESULTS:After heat stress, FCM showed that apoptotic cells were increased significantly and reached the peak at 9 h in Raw 264.7 cells transfected with pcDNA3.1, and were characterized with classical morphologic changes including apoptotic body and nuclear condensation. Agarose gel electrophoresis showed that "DNA ladder" could be observed clearly at 6, 9, and 12 h after the heat stress. But the overexpression of HSF1 could reduce the number of apoptotic cells and inhibit DNA fragmentation.
CONCLUSION:HSF1 can inhibit heat stress-induced apoptosis in Raw264.7 macrophages.
