Specific cell immune response mediated by dendritic cells in multiple myeloma patients in vitro.
- Author:
Hui QI
1
;
Jian-kai SHEN
;
Le XIAO
;
Guang-sen ZHANG
Author Information
1. Department of Hematology, Second Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Dendritic Cells;
immunology;
Granulocyte-Macrophage Colony-Stimulating Factor;
pharmacology;
Humans;
Interleukin-4;
pharmacology;
Monocytes;
cytology;
Multiple Myeloma;
immunology;
pathology;
T-Lymphocytes;
cytology;
T-Lymphocytes, Cytotoxic;
immunology;
Tumor Cells, Cultured
- From:
Journal of Central South University(Medical Sciences)
2006;31(2):222-227
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore KM3 multiple myeloma (MM) cell line specific cytotoxic T lymphocyte (CTL) response in vitro mediated by autologous dendritic cells (DCs) aroused by KM3 cells' lysates and acid-eluted peptides.
METHODS:Monocytes were isolated from the peripheral blood of healthy individuals and MM patients, and were cultured in serum medium with IL-4, GM-CSF, and TNF-alpha to generate DCs. These DCs were pulsed by KM3 cells' lysates or the acid-eluted peptides, then incubated with autologous T lymphocytes for 3 days to induce KM3 cell antigen specific CTL. MTT assay was performed to examine the specific KM3 cells' lysing ability of CTL.
RESULTS:DCs were generated in peripheral blood monocytes cultured in the serum medium containing GM-CSF, IL-4, and TNF-alpha. Autologous T lymphocytes induced by IL-2 and DCs pulsed with KM3 cells' lysates or the acid-eluted peptides showed strong killing effect on KM3 cells.
CONCLUSION:The DCs pulsed by KM3 cells' lysates or the acid-eluted peptides incubated with autologous T lymphocytes can induce KM3 cell antigen specific CTL.
