Sorcin overexpression and multidrug resistance in human gastric cancer cells.
- Author:
Hong YI
1
;
Yi-xuan YANG
;
Can-e TANG
;
Zhu-chu CHEN
;
Gui-ying ZHANG
;
Zhi-qiang XIAO
Author Information
1. Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Calcium-Binding Proteins;
analysis;
biosynthesis;
genetics;
Cell Line, Tumor;
Drug Resistance, Multiple;
Drug Resistance, Neoplasm;
Electrophoresis, Gel, Two-Dimensional;
Humans;
Molecular Sequence Data;
Stomach Neoplasms;
genetics;
metabolism;
Vincristine;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2006;31(3):340-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To screen multidrug resistance (MDR) related proteins in human gastric cancer using proteomics technique.
METHODS:Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant human gastric cancer cell line SGC7901/VCR and its counterpart SGC7901. PDQuest software was used to analyze 2-DE images, and the differential expression proteins between the 2 cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF. The differential expression level of sorcin, one of the identified proteins, was confirmed by western blot analysis. The effect of sorcin on the development of MDR of SGC7901/VCR was determined by antisense oligonucleotides (ASO) technique.
RESULTS:Sorcin as a high expression protein in SGC7901/VCR was identified and the suppression of sorcin expression by sorcin ASO could enhance the vincristine chemosensitivity in SGC7901/VCR.
CONCLUSION:Sorcin overexpression is related to MDR in human gastric cancer cell line SGC7901/VCR.
