Effect of continuously compressive pressure on the expression of RANKL mRNA in human periodontal ligament cells in vitro.
- Author:
Sheng-gao HUANG
1
;
Jian-xing ZHANG
;
Pei-ying XIONG
;
Ming-lang WANG
Author Information
1. Department of Stomatology, Second Xiangya Hospital, Central South University, Changsha 410011, China. huang_shg@126.com
- Publication Type:Journal Article
- MeSH:
Compressive Strength;
Humans;
Periodontal Ligament;
cytology;
metabolism;
RANK Ligand;
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Receptor Activator of Nuclear Factor-kappa B;
genetics;
metabolism;
Stress, Mechanical
- From:
Journal of Central South University(Medical Sciences)
2006;31(4):518-522
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of continuously compressive pressure (CCP) on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) in human periodontal ligament cells (HPDLCs) and to investigate the role of RANKL in alveolar bone rebuilding during orthodontic tooth movement.
METHODS:The primary HPDLCs were isolated from human periodontal ligament by explanting enzymatic digestion with trypsin and collagenase to establish a pressure model. Top-bottom axial pressures (1, 2, and 3 g/cm(2)) were laid on HPDLCs for 0.5, 1.5, 6, 12, 24, and 48 h, respectively. The RANKL expression was identified by the reverse transcription-polymerase chain reaction (RT-PCR) at the mRNA level.
RESULTS:The expression of RANKL mRNA significantly increased in a time-dependent manner (P<0.01), so did the value of pressure, especially in the 2 g/cm(2) group (P<0.05).
CONCLUSION:CCP can up-regulate the expression of RANKL mRNA in human periodontal ligament cells.
