Anti-oxidant effects of Tongxinluo on ATPase in focal brain ischemia-reperfusion rats.
- Author:
Su-ju DING
1
;
Jia-si LI
Author Information
1. Department of Neurology, Changhai Hospital of the Second Military Medical University, Shanghai 200433, China. dingsuju@hotmail.com
- Publication Type:Journal Article
- MeSH:
Adenosine Triphosphatases;
metabolism;
Animals;
Antioxidants;
pharmacology;
Brain Ischemia;
enzymology;
Drugs, Chinese Herbal;
pharmacology;
Glutathione Peroxidase;
metabolism;
Male;
Malondialdehyde;
metabolism;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
enzymology;
Superoxide Dismutase;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2006;31(4):552-555
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of Tongxinluo on adenosine triphosphatase (ATPase), anti-oxidant enzymes activities, and lipid peroxidation of mitochondria or brain homogenate in focal brain ischemia-reperfusion rats.
METHODS:The models of the focal brain ischemia-reperfusion rats were made by the middle cerebral artery occlusion (MCAO). Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and ATPase and malonaldehyde (MDA) levels of mitochondria or brain homogenate were measured by biochemical methods.
RESULTS:Tongxinluo significantly inhibited the decrease of activities of SOD and the increase of MDA levels, but had no difference in GSH-Px in brain homogenate. It also inhibited the decrease of activities of SOD, GSH-Px, ATPase, and the increase of MDA levels in mitochondria.
CONCLUSION:The protective mechanisms of Tongxinluo against mitochondrial injuries in focal ischemia-reperfusion rats may be derived from reducing lipid peroxides, scavenging free radicals and improving the energy metabolism.
