Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength detection.
- Author:
Jian-ping SONG
1
Author Information
1. Department of Pharmacy, Hunan Tumor Hospital, Changsha 410006, China. sjping333@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
methods;
Humans;
Reproducibility of Results;
Rhodamine 123;
analysis;
Spectrophotometry;
methods
- From:
Journal of Central South University(Medical Sciences)
2006;31(4):610-612
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a high-performance liquid chromatography with visible wavelength detection method for rhodamine 123 in cell lysate.
METHODS:The HPLC separation was performed on a Kromasil C(18) (5 microm, 4.6 mm x 250 mm) column, using water (15 mmol/L potassium acetate and 1.2 mmol/L tetrabutylammonium bromide) - acetonitrile (65:35) as the mobile phase. The wave length was 390 nm, the internal standard was rhodamine B. Protein in the sample was precipitated by trichloroacetic acid.
RESULTS:The calibration curve was linear in the range of 3 - 300 microg/L. The intra-day and inter-day RSDs were less than 5%.
CONCLUSION:The method is accurate,sensitive, simple, and reliable for determining rhodamine 123 in cell lysate.
