Protective effect of resveratrol against oxidative damage of UVA irradiated HaCaT cells.
- Author:
Ming-liang CHEN
1
;
Ji LI
;
Wei-rong XIAO
;
Lei SUN
;
Hua TANG
;
Lin WANG
;
Ling-yan WU
;
Xiang CHEN
;
Hong-fu XIE
Author Information
1. Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Transformed;
DNA Damage;
radiation effects;
Glutathione Peroxidase;
metabolism;
Humans;
Keratinocytes;
cytology;
metabolism;
radiation effects;
Malondialdehyde;
metabolism;
Oxidative Stress;
Radiation-Protective Agents;
pharmacology;
Resveratrol;
Stilbenes;
pharmacology;
Superoxide Dismutase;
metabolism;
Ultraviolet Rays
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):635-639
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To observe the photoprotective effect and possible mechanisms of resveratrol for ultraviolet A (UVA) irradiated HaCaT cells.
METHODS:HaCaT cells under UVA irradiation with 5J/cm(2) were interfered with 0.01 mmol/L and 0.1 mmol/L resveratrol. The testing objects were divided into a control and a UVA irradiation group, and then we detected the proliferation capacity with methylthiazdyl tetrazolium (MTT) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, content of maleic dialdehyde (MDA) with hydroxylamine, colorimetric, thiobarbituric acid (TBA) methods. The ultrastructure was observed under electron microscope.
RESULTS:Resveratrol could enhance the proliferation activity, SOD, GSH-Px activity of HaCaT cells under UVA irradiation, decrease the content of MDA in dose-dependent manner (P<0.05). The electron microscope revealed that resveratrol could relieve the injury of HaCaT cells' ultrastructure.
CONCLUSION:Resveratrol can relieve the inhibition to HaCaT cell proliferation,injury of their ultrastructure and oxidation by UVA irradiation. The protection is dose-dependent. That resveratrol raises the oxidase activity and clears the oxyradical may account for these results.
