Inhibitory effect of reinioside C on LOX-1 expression induced by ox-LDL.
- Author:
Yong-ping BAI
1
;
Guo-gang ZHANG
;
Rui-zheng SHI
;
Yuan-jian LI
;
Gui-shan TAN
;
Jia CHEN
Author Information
1. Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Drugs, Chinese Herbal;
pharmacology;
Endothelium, Vascular;
Humans;
Lipoproteins, LDL;
pharmacology;
Polygala;
chemistry;
RNA, Messenger;
biosynthesis;
genetics;
Receptors, LDL;
biosynthesis;
genetics;
Saponins;
pharmacology;
Umbilical Veins;
cytology;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):659-662
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of reinioside C (RC) on the expression of lectin-like oxidized low density lipoprotein receptor (LOX)-1 mRNA and LOX-1 protein induced by oxidized low density lipoprotein (ox-LDL) in cultured human umbilical vein endothelial cells (HUVEC).
METHODS:HUVECs were cultured with ox-LDL (50 mg/L) for 24 h in the absence or presence of RC (1, 3, and 10 micromol/L). The expressions of LOX-1 mRNA and LOX-1 protein were examined by RT-PCR and Western-blot.
RESULTS:Incubation with ox-LDL (50 mg/L) significantly raised the expression of LOX-1 mRNA and LOX-1 protein,which was concentration-dependent.
CONCLUSION:RC can inhibit the increased expression of LOX-1 mRNA and LOX-1 protein induced by ox-LDL in HUVECs.
