P21 expression in renal interstitial fibrosis and regulative effect of enalapril.
- Author:
Yun XIAO
1
;
Li-jian TAO
;
Tang DAMU
;
Ou JIN
;
Jian-hua ZHOU
;
Ming SHEN
;
Jing HU
;
Chun-yan LIU
;
Jian SUN
;
Wang-bin NING
Author Information
1. Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Angiotensin-Converting Enzyme Inhibitors;
pharmacology;
therapeutic use;
Animals;
Enalapril;
pharmacology;
therapeutic use;
Kidney Tubules;
metabolism;
Male;
Nephrosclerosis;
drug therapy;
metabolism;
Proto-Oncogene Proteins p21(ras);
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Ureteral Obstruction;
complications
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):663-670
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of P21 in renal interstitial fibrosis rats and the effect of enalapril on it.
METHODS:Sprague Dawley rats were randomly divided into 3 groups: a sham operation group,a unilateral urethral obstruction group, and an enalapril treatment group. The expression of P21 in renal tubular epithelial cells on the process was detected by immunohistochemistry at different time spots (7, 14, 21 d after UUO, sham-surgery or enalapril treatment). The expression of p21 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS:Seven days after the surgery, significant differences were found in P21 expression between UUO and SOR renal tubular cells. With degree of interstitial fibrosis aggravating, P21 expression increased. Enalapril can inhibit its expression.
CONCLUSION:In the kidney of UUO rats, P21 expression increased and enalapril possessed significant inhibitory effects on the procedure. P21 may participate in the pathogenesis of renal tubule-interstitial fibrosis.
