Killing effect of VP3 on human nasopharyngeal carcinoma cell line CNE-2 cells.
- Author:
Jing XU
1
;
Yuan-zheng QIU
;
Yao-yun TANG
;
Yong-quan TIAN
;
Xian-zhong XIAO
;
Su-ping ZHAO
Author Information
1. Department of Otolaryngology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Base Sequence;
Capsid Proteins;
genetics;
physiology;
Cell Line, Tumor;
Genetic Therapy;
Humans;
Molecular Sequence Data;
Nasopharyngeal Neoplasms;
pathology;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):706-709
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the killing effects of VP(3) on nasopharyngeal carcinoma cell line CNE-2.
METHODS:Plasmid expression vector pcDNA3.1(-) CMV.VP(3)-His was constructed and identified by Kpn I/EcoR I endonuclease analysis, and then sequenced to verify successful insertion in the sense direction of VP(3) gene. pcDNA3.1(-) CMV.VP(3)-His and pcDNA3.1(-)-His expression plasmid was transiently transfected into nasopharyngeal carcinoma cell line CNE-2 . VP(3) protein expression was detected by Western blotting. MTT assay was used to determine the killing effects of VP(3) gene on nasopharyngeal carcinoma cell line CNE-2.
RESULTS:Endonuclease analysis and sequencing confirmed the recombinant plasmid contained the complete VP(3) CDS sequence. Western blotting detected a 14.03 kD protein expression from the transfected cells, which was the expecting band of VP(3) gene. The growth of CNE-2 cells that expressed VP(3) gene was inhibited,while the growth of CNE-2 cells that did not express VP(3) gene was not inhibited.
CONCLUSION:VP(3) gene can kill nasopharyngeal carcinoma cell CNE-2.
