Apoptosis of breast cancer cell line MCF-7 cells induced by gemcitabine and radiation.
- Author:
Jian-huang LI
1
;
Xue-gang LUO
Author Information
1. Department of Oncology, Xiangya Hospital, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Antimetabolites, Antineoplastic;
pharmacology;
Apoptosis;
drug effects;
radiation effects;
Breast Neoplasms;
pathology;
Deoxycytidine;
analogs & derivatives;
pharmacology;
Female;
Flow Cytometry;
Humans;
Radiation;
Radiation-Sensitizing Agents;
pharmacology;
Tumor Cells, Cultured
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):710-713
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the apoptosis of human breast cell line MCF-7 cells induced by gemcitabine and radiation.
METHODS:The MTT method was applied to study the growth inhibition of MCF-7 treated with gemcitabine, radiation, gemcitabine and radiation. The apoptosis index (AI) was analyzed by flow cytometry. The morphology of the MCF-7 cells apoptosis was observed by transmission electron microscopy.
RESULTS:When MCF-7 cells were treated with gemcitabine at different concentrations for 24 h, the cell growth inhibition rate was increased in a concentration-dependent manner. The apoptotic indexes (AI) of MCF-7 of four groups by flow cytometry revealed. The AI of (R+D) group was significantly different from those of the radiation group and the gemcitabine group (P<0.05). Condensed chromation, nuclear fragmentation and apoptotic body of MCF-7 cells were found by transmission electron microscope.
CONCLUSION:The apoptosis of human breast cancer cell line, MCF-7 cells, could be induced by gemcitabine. Gemcitabine can significantly enhance the radiation-induced apoptosis of MCF-7 cells.
