Effects of matrix metalloproteinase-3, osteopontin, and tissue inhibitor of metalloproteinase-1 in the formation of cataract.
- Author:
Qian TAN
1
;
Jian JIANG
;
Dong-qiang LUO
Author Information
1. Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China. Liteliu@163.net
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Cataract;
classification;
etiology;
metabolism;
Epithelial Cells;
metabolism;
Female;
Humans;
Lens, Crystalline;
metabolism;
Male;
Matrix Metalloproteinase 3;
biosynthesis;
Middle Aged;
Osteopontin;
biosynthesis;
Tissue Inhibitor of Metalloproteinase-1;
biosynthesis
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):737-741
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and significance of matrix metalloproteinase-3 (MMP-3), osteopontin (OPN) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in human lens epithelial cells (LECs) of cataract.
METHODS:The MMP-3, OPN, and TIMP-1 expressions in LECs of anterior subcapsular cataract (31 cases), nuclear cataract (28 cases) and normal lens (12 cases) were detected by immunohistochemistry, respectively.
RESULTS:The MMP-3 expression in anterior subcapsular cataract was significantly higher than that in nuclear cataract and normal lens (chi2=31.49, 34.479; P=0.000); but there was no statistic significance between nuclear cataract and normal lens (chi2=2.449, P=0.118). The OPN expression in anterior subcapsular cataract was also significantly higher than that in nuclear cataract and normal lens (chi2=29.450, 15.889; P=0.000). There was no significant difference in the TIMP-1 expression among the 3 groups (P>0.05). Positive correlation was found between MMP-3 and OPN in LECs of anterior subcapsular cataract (r=0.381, P=0.035). But no significant correlation was found among MMP-3, OPN, and TIMP-1 (r=0.121, -0.289; P=0.516, 0.114).
CONCLUSION:Increased expression of MMP-3 and OPN and the expression imbalance between MMP-3 and TIMP-1 may play a critical role in the pathogenesis of anterior subcapsular cataract.
