Determination of diltiazem hydrochloride injection by high-performance liquid chromatography.
- Author:
Ling TANG
1
;
Ting ZHANG
;
Xin-zhong LI
Author Information
1. Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Calcium Channel Blockers;
analysis;
Chromatography, High Pressure Liquid;
Diltiazem;
analysis
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):756-758
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an high-performance liquid chromatography (HPLC) method for the determination of diltiazem hydrochloride injection.
METHODS:The assay was conducted on a NUCLEODUR C18 column with buffer solution (pH6.2)-acetonitrile-methanol (40:30:30) as the mobile phase and the detection wavelength was 240 nm.
RESULTS:Diltiazem hydrochloride and relevant substances could be separated under the condition. The linear concentration range of diltiazem hydrochloride was 4 - 200mg/L with the recovery of 100.22% (RSD = 0.37%).
CONCLUSION:The method may be used for the determination of diltiazem hydrochloride injection.
