Angiogenic effect of bone marrow mesenchymal stem cells transfected with human VEGF gene on myocardial infarcts in rats.
- Author:
Wen-wu ZHOU
1
;
Jian-guo HU
;
Jin-fu YANG
;
Ling LIN
;
Xin-min ZHOU
;
Tao TANG
Author Information
1. Department of Cardiothoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Coronary Circulation;
Humans;
Male;
Mesenchymal Stem Cell Transplantation;
Mesenchymal Stem Cells;
metabolism;
Myocardial Infarction;
metabolism;
pathology;
Neovascularization, Physiologic;
Rats;
Rats, Wistar;
Transfection;
Vascular Endothelial Growth Factor A;
genetics
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):763-771
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the angiogenic effect of the bone marrow mesenchymal stem cells (MSCs) transfected with human vascular endothelial growth factor (VEGF(165)) gene on myocardial infarcts in rats.
METHODS:The animal model of heart ischemic was established by ligating the left anterior descending coronary artery in Wistar rats. The ligated rats were divided into 4 groups (n=12 each), and 2 weeks later they were injected hVEGF-transfected MSC at the heart infarct zone (Group A), MSC (Group B), liposome-hVEGF gene plasmid (Group C), and medium (Group D). Four weeks after the injection, the capillary density of the infracted zone and the expression of human VEGF in vivo were examined.
RESULTS:Four weeks after the transplantation,the capillary density was significantly greater in Group A than that in Group B and Group D, slightly greater than that in Group C. The highest expression of hVEGF was Group A, and followed by Group C, Group B, and Group D.
CONCLUSION:MSC is helpful for the stable expression of hVEGF gene, and is an ideal cellular vehicle for VEGF genes.
