Effect of chronic stress on PKA and P-CREB expression in hippocampus of rats and the antagonism of antidepressors.
- Author:
Zhe WANG
1
;
Sui-yu HU
;
De-liang LEI
;
Wei-xi SONG
Author Information
1. Department of Combined Traditional Chinese Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China. ericwangzhe@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Antidepressive Agents, Second-Generation;
antagonists & inhibitors;
Cyclic AMP Response Element-Binding Protein;
biosynthesis;
genetics;
Cyclic AMP-Dependent Protein Kinases;
biosynthesis;
genetics;
Depression;
etiology;
metabolism;
Fluoxetine;
antagonists & inhibitors;
Hippocampus;
metabolism;
Male;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Stress, Physiological;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2006;31(5):767-771
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of chronic unpredicted sequence of mild stress on the expression of cAMP-dependent protein kinase A(PKA) and phosphorylated cAMP-responsive element binding protein (P-CREB) in hippocampus of rats and the antagonism of antidepressors (fluoxetine).
METHODS:Thirty-six male Sprague Dawley rats were randomly and equally allocated to 3 groups: A normal control group, a model group, and a fluoxetine group. All rats except the control group were singly housed and exposed to an unpredicted sequence of mild stressors. The different distribution and expression of PKA and P-CREB in the hippocampus of rats in different groups were investigated with immunohistochemistry and Westernblot technique.
RESULTS:The positive PKA and P-CREB cells in the hippocampus of normal controls were the pyramidal cells and the granule cells. The PKA and P-CREB protein expression levels in the hippocampus of model rats were significantly lower than those of the normal controls (P<0.05). The PKA and P-CREB protein expression levels in the hippocampus of the fluoxetine group were significantly higher than those of the model group (P<0.05).
CONCLUSION:Chronic unpredicted mild stress can affect the PKA and P-CREB expression in hippocampus of rats and fluoxetine has antagonism against it.
