Porcine endogenous retrovirus in Daweizi pigs in Hunan.
- Author:
Xiao-Wei XING
1
;
Li-Qun XUE
;
Zhao-Hui MO
;
Sheng-Qiang HUANG
;
Wei WANG
Author Information
1. Cell Transplantation & Gene Therapy Center, Third Xiangya Hospital of Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Animals;
DNA, Viral;
isolation & purification;
Endogenous Retroviruses;
isolation & purification;
Humans;
Polymerase Chain Reaction;
methods;
Swine;
virology;
Transplantation, Heterologous
- From:
Journal of Central South University(Medical Sciences)
2006;31(6):838-842
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect porcine endogenous retrovirus (PERV) in Daweizi pigs and to provide basic parameters of evaluating the biological safety for xenotransplantation from pigs to humans.
METHODS:Ear tissues from 42 individuals were randomly collected from a Daweizi pig population. PCR and RT-PCR were performed to detect PERV proviral DNA and mRNA respectively. Finally, env-A, env-B, and env-C were amplified, sequenced, and analyzed using the BLAST software in National Center for Biotechnology Information.
RESULTS:PERV proviral DNA and mRNA could be detected in the 42 individuals by PCR and RT-PCR, respectively. env-A, env-B and env-C were detected in all the individuals. Compared with other pig species (AY288779, DQ011794 and AY534304), there was 1 and 8 bp differences in the sequences of env-A and env-C, while no difference in env-B.
CONCLUSION:PERV exists and has transcriptive activity in Daweizi pigs. The predominate subtype is PERV-ABC. Env genes are firstly cloned and sequenced in Daweizi pigs and there are polymorphism in the breed. As to the biological safety, the breed was not suitable as a donor in xenotransplantation.
