In vitro study on the differentiation of bone marrow mesenchymal stem cells into epidermal stem cells.
- Author:
Jian-hong LONG
1
;
Fang-fen LIU
;
Min QI
Author Information
1. Department of Burns and Plastics, Xiangya Hospital, Central South University, Changsha 410008, China. jianhong.long@gmail.com
- Publication Type:Journal Article
- MeSH:
Adult;
Bone Marrow Cells;
cytology;
Cell Culture Techniques;
Cell Differentiation;
Cells, Cultured;
Epithelial Cells;
cytology;
Female;
Humans;
Male;
Mesenchymal Stem Cells;
cytology;
Stem Cells;
cytology;
Young Adult
- From:
Journal of Central South University(Medical Sciences)
2006;31(6):866-871
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the feasibility of the differentiation of bone marrow mesenchymal stem cells into epidermal stem cells.
METHODS:Human bone mesenchymal stem cells (BMSCs) were isolated from the bone marrow of volunteers' ilium, and were purified and cultured in vitro and induced by supernatant liquid of HaCat cells. The morphologic changes of BMSCs were observed with electron microscope, and immunohistochemical examination was carried out to detect the positive staining of cytokeratin 10, cytokeratin 19 and integrin beta1 after one week induction.
RESULTS:Large numbers of flat-polygon cells were closely connected and developed like road metal. The plenty threadlike keratins were observed in the cytoplasm. The immunohistochemical examination showed the positive staining of few cytokeratin 10, a great deal of cytokeratin 19 and integrin beta1 after the one week induction.
CONCLUSION:Human BMSCs can differentiate into epidermal stem cells with supernatant liquid of HaCat cells and EGFs in vitro.
