Effects of advanced glycation end products on the activity of NF-kappaB and the expression of fibronectin mRNA in the endothelial cells in aged rats.
- Author:
Xiu-mei XIE
1
;
Zhi-wei YANG
;
Mei-fang CHEN
Author Information
1. Department of Geriatric Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. chenmeifang@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Endothelial Cells;
drug effects;
metabolism;
Fibronectins;
genetics;
metabolism;
Glycation End Products, Advanced;
pharmacology;
NF-kappa B;
genetics;
metabolism;
RNA, Messenger;
genetics;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2006;31(6):883-887
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of advanced glycation end products (AGEs) on the activity of NF-kappaB and fibronectin (Fn) synthesis in the endothelial cells in aged rats.
METHODS:Endothelial cells were cultured in M199 from the aorta of 24 month old rats and divided into 3 groups: Group A (5 mmol/L glucose) as controls, Group B (25 mg/L AGEs for 48 h), and Group C (50 mg/L AGEs for 48 h). The activity of NF-kappaB was evaluated by immunofluorescence and the expression of Fn mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS:Compared with the controls, AGEs induced the activity of NF-kappaB and increased the Fn mRNA expression in a concentration-dependent manner (P<0.05).
CONCLUSION:The activity of NF-kappaB and up-regulated expression of Fn mRNA induced by AGEs may contribute to chronic complications of diabetes.
