Qualitative detection of hepatitis C virus core antigen in the serum in patients with chronic hepatitis C.
- Author:
Yi OUYANG
1
;
De-ming TAN
;
Tie-gang LI
;
Hui ZHOU
;
Chang TAN
Author Information
1. Departerment of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Hepatitis C Antigens;
blood;
Hepatitis C, Chronic;
blood;
Humans;
RNA, Viral;
blood;
Viral Core Proteins;
blood
- From:
Journal of Central South University(Medical Sciences)
2006;31(6):894-905
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the clinical significance of serum hepatitis C virus (HCV) core antigen detected by enzyme linked immunosorbent assay (ELISA).
METHODS:The serum HCV core antigen, which was taken from 149 patients with chronic hepatitis C, 20 patients of chronic hepatitis B and 20 health volunteers, was detected by ELISA. Meanwhile, the serum HCV RNA was detected by RT-PCR, and anti-HCV was detected by ELISA.
RESULTS:The qualitative HCV core antigen in the serum, which was take from 20 patients of chronic hepatitis B and 20 health volunteers, was negative.The positive percentage of HCV core antigen was 49.66% in the 149 sera of patients with chronic hepatitis C. The coincidence of detective results of HCV RNA and HCV core antigen was 54.36%, without significant difference (P>0.05). The positive percentage of HCV RNA and HCV core antigen in the 149 anti-HCV antibody positive sera samples were 55.03% (82/149) and 49.66% (74/149), respectively, and there was no significant difference (P>0.05).
CONCLUSION:The qualitative HCV core antigen detected by ELISA has a high specificity. The positive percentage of HCV core antigen in the serum of patients with chronic hepatitis C is 49.66%. HCV core antigen is related to HCV RNA. HCV core antigen may be a useful serum marker which could show HCV viraemia like HCV RNA.
