Cloning and Expression of the Gene Encoding the 32-kDa Protein of Rickettsia typhi.
- Author:
Kyung Hee PARK
;
Myong Joon HAHN
;
Jong Hyun KIM
- Publication Type:Original Article
- MeSH:
Clone Cells*;
Cloning, Organism*;
Crystallins;
Humans;
Maltose-Binding Proteins;
Open Reading Frames;
Recombinant Proteins;
Rickettsia typhi*;
Rickettsia*;
Sequence Analysis;
Typhus, Endemic Flea-Borne
- From:Journal of the Korean Society for Microbiology
1997;32(4):399-404
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The crystalline surface layer protein (SLP) and a 28-32 kDa antigen of Rickettsia typhi were known as strong immunogens. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32-kDa protein. Our study also showed that a 48-kDa protein reacted strongly with polyclonal antiserum of a patient with murine typhus. In this study, we produced three recombinant proteins (SLP, 32-kDa, and 48-kDa protein) in E. coli as fusion proteins with maltose binding proteins. The reactivity of these proteins with patients' sera was investigated.
