Significance and expression of cyclooxygenase-2 mRNA in peripheral blood monocytes in patients with acute coronary syndrome.
- Author:
Ping DENG
1
;
Shui-ping ZHAO
;
Hong-guang HUANG
;
Jie WU
;
Jiang LI
;
Hong-nian ZHOU
Author Information
1. Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Aged;
Angina Pectoris;
blood;
enzymology;
Coronary Artery Disease;
blood;
enzymology;
Cyclooxygenase 2;
biosynthesis;
genetics;
Female;
Humans;
Interleukin-6;
biosynthesis;
Male;
Matrix Metalloproteinase 9;
biosynthesis;
Middle Aged;
Monocytes;
enzymology;
RNA, Messenger;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2005;30(4):403-406
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the expression of cyclooxygenase-2 (COX-2) mRNA in peripheral blood monocytes in patients with acute coronary syndrome (ACS), and to explore the effect of the expression of COX-2 mRNA in ACS.
METHODS:The expressions of COX-2 mRNA in peripheral blood monocytes from 18 normal subjects and 42 ACS patients were analyzed by reverse transcription polymerase chain reaction (RT-PCR), and the monocytes from patients were incubated with celecoxib in vitro. The concentrations of interleukin-6 (IL-6) and matrix metalloproteinase-9 (MMP-9) in supernates of monocytes were measured by enzyme-linked immunosorbent assays (ELISA).
RESULTS:The expression of COX-2 mRNA and the secrections of IL-6 and MMP-9 in peripheral blood monocytes in ACS patients significantly increased compared with those from normal controls [0.61 +/- 0.17 vs 0.11 +/- 0.09; (97.24 +/- 11.21) ng/L vs (22.15 +/- 6.30) ng/L; (41.20 +/- 8.41) g/L vs (11.76 +/- 4.23) g/L; all P < 0.05, respectively]. Celecoxib reduced IL-6 and MMP-9 secretion level of monocytes from ACS patients up to 48% and 50% respectively (all P < 0.05), in a concentration-dependent manner.
CONCLUSION:COX-2 in peripheral blood monocytes may play an important role in the acute coronary syndrome.
