Preparation and analysis of monoclonal antibody against EphA4 peptide.
- Author:
Jun-Jie YANG
1
;
Weimin QI
Author Information
1. Departnent of Hematology, Second Xiangya Hospital, Central South University, Changsha 410011, China. zmyang126@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
biosynthesis;
Hybridomas;
Mice;
Mice, Inbred BALB C;
Peptide Fragments;
immunology;
Receptor, EphA4;
immunology
- From:
Journal of Central South University(Medical Sciences)
2005;30(5):529-532
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare monoclonal antibody (MAb) against carboxy-terminus of E-phA4 and to analyze the immunological characteristics and significance of the antibody.
METHODS:Mice were immunized with a chemically synthesized peptide that had been conjugated to KLH (keyhole limpet hemocyamin). A panel of antibodies specifically against EphA4 were obtained by hybridoma technique. The immunological properties and significance of the MAb were analyzed by immunological and immunochemistry techniques.
RESULTS:A hybridoma cell line secreting anti-EphA4 MAb was established. The MAb reacted specifically with human, chicken and mouse EphA4, but did not cross-react with EphA5 and EphA7. The antibody could be used for immunochemical techniques such as ELISA, immunoprecipitation, Western blot and immunohistochemistry.
CONCLUSION:The anti-EphA4 MAb generated by immunizing a synthetic peptide possesses excellent immunological properties. MAb can be applied for immunological and immunohistochemical purpose and will become an important tool in the study of EphA4 and its ligand.
