Effect of Bcl-2 antisense phosphorothioate oligodeoxynucleotides on bile duct carcinoma cell line QBC939.
- Author:
Chuang PENG
1
;
Hui-huan TANG
;
Ping HUANG
;
Ming-xiong CHEN
Author Information
1. Department of General Surgery, Xiangya Hospital, Central South University Changsha, China. tanghuihuan@hotmail.com
- Publication Type:Journal Article
- MeSH:
Bile Duct Neoplasms;
metabolism;
pathology;
Cell Division;
drug effects;
Humans;
Lipids;
pharmacology;
Oligonucleotides, Antisense;
pharmacology;
Proto-Oncogene Proteins c-bcl-2;
biosynthesis;
genetics;
Transfection;
Tumor Cells, Cultured;
Tumor Stem Cell Assay
- From:
Journal of Central South University(Medical Sciences)
2005;30(5):536-539
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of Bcl-2 antisense oligodeoxynucleotides (ASODN) on the cell proliferation and Bcl-2 expression in bile duct carcinoma cell line QBC939.
METHODS:Bcl-2 ASODN and control sequence were transfected into cell line QBC939 by Lipofectamine 2000. The changes of Bcl-2 protein were detected by Western blot. The survival rate and colony formation rate of QBC939 cells incubating with Bcl-2 ASODN were evaluated by trypan blue staining assay and colony forming test.
RESULTS:The densitometric analysis of Gel-photograph showed that the level of Bcl-2 protein expression in the ASODN transfected group was significantly lower than that in the controls (P < 0.01). Both trypan blue staining assay and colony forming test demonstrated that Bcl-2 ASODN could partially inhibit the growth of QBC939 cells. After incubating with Bcl-2 ASODN, the survival rate and colony formation rate of QBC939 cells were significantly lower than those of the controls (P < 0.05).
CONCLUSION:Bcl-2 ASODN inhibits the cell proliferation in bile duct carcinoma cell line QBC939 by blocking the expression of bcl-2 gene.
