Effect of neferine on the chemotherapic sensitivity of STI 571 to K562/A02 cells.
- Author:
Xi-Bin XIAO
1
;
Zhao-Xia XIE
;
Jie CHEN
;
Qun QIN
;
Yan ZHU
Author Information
1. Department of Hematology, Xiangya Hospital, Central South University, Changsha, China. xzxchangsha@163.com
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter, Subfamily B, Member 1;
biosynthesis;
genetics;
Antineoplastic Agents, Phytogenic;
pharmacology;
Benzamides;
Benzylisoquinolines;
pharmacology;
Cell Proliferation;
Drug Resistance, Multiple;
Drug Resistance, Neoplasm;
Drug Synergism;
Humans;
Imatinib Mesylate;
K562 Cells;
Piperazines;
pharmacology;
Protein-Tyrosine Kinases;
antagonists & inhibitors;
Pyrimidines;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2005;30(5):558-561
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of neferine on the chemotherapic sensitivity of STI 571 to K562/A02 cells and to reveal its mechanism.
METHODS:MTT method was used to observe the alteration of the proliferation of K562/A02 cell line treated with STI571 alone or combined with neferine. The transcription of mdrl gene was detected by semi-quantitative RT-PCR and the P-gp expression was determined by Western blot after STI 571 alone or combined with neferine treatment.
RESULTS:The cytotoxic effect of STI 571 (1 micromol/L) combined with neferine (IC50 = 3.02 micromol/L) on K562/A02 cell line was significantly higher than that of STI 571 alone (IC50 = 0.689 micromol/L). After treating with STI571 combined with neferine, the synergistic interaction on K562/A02 cells increased 4.38 folds (P < 0.05); the mdrl mRNA expression by semi-quantitative RT-PCR was significantly reduced by (45.4 +/- 2.5) % (P < 0.01); and the P-gp expression by Western blot was deregulated by 40.58% (P < 0.05).
CONCLUSION:Neferine significantly enhances the antineoplastic effect of STI 571 on K562/A02 cells by reducing mdrl mRNA transcription and blocking P-gp expression.
