Isolation, induction and differentiation of human blood-derived endothelia progenitor cells.
- Author:
Ze-Hao LIU
1
;
Min-Xiang LEI
;
Ai-Min WANG
;
Zuo-Xiong CHEN
;
Jun ZHANG
;
San-E TANG
Author Information
1. Departement of Endocrinology, Xiangya Hospital, Xiangya Hospital, Central South University, Changsha, China. medicalliu@hotmail.com
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cell Separation;
Endothelial Cells;
cytology;
Endothelium, Vascular;
cytology;
Humans;
Leukocytes, Mononuclear;
cytology;
Stem Cells;
cytology
- From:
Journal of Central South University(Medical Sciences)
2005;30(5):566-569
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the biological traits and optimal condition for the induction and differentiation of endothelial progenitor cells from peripheral blood in healthy adults.
METHODS:Mononuclear cells isolated from peripheral blood of healthy adults were cultured in M199 medium supplemented with VEGF, bFGF, IGF-1, and EGF. The appearing time of cell clusters or spindle-shaped cells was recorded respectively. Attached spindle-shaped cells were detached and labeled with a series of antibodies against blood vessel endothelial-specific markers.
RESULTS:Attached spindle-like cells appeared 4 days after the culture, cell clusters were observed at 5 to 8 days, and cord-like structure was formed by 10th day. These cells expressed endothelial-specific markers.
CONCLUSION:Endothelial progenitors cells were derived from mononuclear cells of peripheral blood, which can be induced into endothelial cells at specific conditions.
