Rapid determination of optimal time for peripheral blood stem cell harvest by detecting hematopoietor progenitor cell during mobilization.
- Author:
Jing-Jing GUO
1
;
Si-Yun WU
;
Yuan-Lin CHEN
Author Information
1. Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, China. cscy1168@sina.com
- Publication Type:Journal Article
- MeSH:
Adult;
Cell Count;
Female;
Granulocyte Colony-Stimulating Factor;
therapeutic use;
Hematopoietic Stem Cell Mobilization;
methods;
Hematopoietic Stem Cell Transplantation;
methods;
Hematopoietic Stem Cells;
cytology;
Humans;
Male;
Recombinant Proteins;
Time Factors
- From:
Journal of Central South University(Medical Sciences)
2005;30(5):570-573
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a simple method to rapidly determine the optimal time of perpheral blood stem cell harvest.
METHODS:We selected 5 healthy donors mobilized with granulocyte colony-stimulating factor (G-CSF) in allogenic peripheral blood stem cell transplantation (allo-PBSCT) to continuously monitor the variety of hematopoietic stem/progenitor cells (HSC/HPC) in the peripheral blood. HPC in the peripheral blood was detected by Sysmex XE-2100 fully automated hematology analyzer with an immature myeloid information channel and the CD34+ cells and colony-forming unit-granulocyte-macrophage (CFU-GM) were determined by flow cytometry and colony culture during mobilization.
RESULTS:HPC was positively correlated with the CD34+ cells or CFU-GM in the peripheral blood (r = 0.82 or r = 0.85, respectively). HPC, CD34+ cells, and CFU-GM in allo-PBSCT donors obviously increased on the 4th day after the mobilization, and their peaks were on the 5th day after the mobilization. But HPC varied more than CD34+ cells and CFU-GM did.
CONCLUSION:Sysmex XE-2100 can rapidly monitor the variety of HSC/HPC in allo-PBSCT donors mobilizated with G-CSF, and can provide a simple and economic method to determine the optimal time of peripheral blood stem cell harvest.
