Effect of indomethacin on fibronectin and plasminogen activator inhibitor-1 in cultured human peritoneal mesothelial cells.
- Author:
Xin LIU
1
;
You-Ming PENG
;
Fu-You LIU
;
Jia-Li WEI
Author Information
1. Department of Nephrology, First Hospital, Tsinghua Universiiy, Beijing, China. felh@medmail.com.cn
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Cyclooxygenase Inhibitors;
pharmacology;
Epithelial Cells;
metabolism;
Fibronectins;
metabolism;
Humans;
Indomethacin;
pharmacology;
Peritoneum;
cytology;
metabolism;
Plasminogen Activator Inhibitor 1;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2005;30(5):590-603
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of indomethacin on high concentration glucose and lipopolysaccharide (LPS) induced fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) secretion in cultured human peritoneal mesothelial cells.
METHODS:Mesothelial cells were isolated from human omental specimens by trypsin disaggregation and incubated by 2.5% glucose or LPS together with different concentrations of indomethacin. Enzyme-linked immunosorbent assay determined the quantity of FN and PAI-1 in the cultured supernatants.
RESULTS:Compared with the control group, the levels of FN and PAI-1 in the cultured supernatants were increased significantly exposuring to high concentration glucose and LPS (P <0.01). The different concentrations of indomethacin decreased FN and PAI-1 secretion in the 2.5% glucose or the LPS group within 24 h (P < 0.05).
CONCLUSION:Indomethacin can inhibit the synthesis and secretion of extracellular matrix in human peritoneal mesothelial cells, which may be effective in the gene therapy for peritoneal fibrosis.
