Effect of xiaokeling concentration fluid on mRNA expression of insulin-like growth factor-1 in sciatic nerve of Streptozotocin-induced diabetic rats.
- Author:
Jiang-zheng ZENG
1
;
Ke-li DONG
;
Guang-cheng LI
;
Liang-ming LI
Author Information
1. Department of Traditional Chinese Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China. zjzxyfs2003@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Diabetes Mellitus, Experimental;
drug therapy;
metabolism;
Diabetic Neuropathies;
prevention & control;
Drugs, Chinese Herbal;
pharmacology;
therapeutic use;
Hypoglycemic Agents;
pharmacology;
therapeutic use;
Insulin-Like Growth Factor I;
biosynthesis;
genetics;
Male;
RNA, Messenger;
biosynthesis;
genetics;
Random Allocation;
Rats;
Receptor, IGF Type 1;
Reverse Transcriptase Polymerase Chain Reaction;
Sciatic Nerve;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2005;30(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of xiaokeling concentration fluid on insulin-like growth factor-1 (IGF-1) mRNA expression in sciatic nerve of Streptozotocin-induced diabetic rats.
METHODS:Thirty diabetic rats were randomly divided into model group, mecobalamin tablets group, and xiaokeling concentration fluid group. The IGF-1 mRNA level in sciatic nerve of each group was determined after 8 weeks by relative quantity RT-PCR.
RESULTS:The IGF-1 mRNA level in sciatic nerve of diabetic rats between xiaokeling concentration fluid group, mecobalamin tablets group and normal group showed no significant difference ( P = 0.213, P = 0.822, P = 0.304 ), while was significantly higher than that of the model group ( P < 0.05 ). IGF-1 mRNA level was negatively correlated with the level of blood sugar (P < 0.05).
CONCLUSION:IGF-1 mRNA level decreased in sciatic nerve of diabetic rats. Xiaokeling concentration fluid can increase the IGF-1 mRNA level in sciatic nerve of diabetic rats. Xiaokeling concentration fluid is involved in the regulation of IGF-1 expression, and probably prevents diabetic peripheral neuropathy from deteriorating.
