Expression and location of UBAP1 protein associated with nasopharyngeal carcinoma.
- Author:
Zhao-yang ZENG
1
;
Jun QIAN
;
Wei XIONG
;
Yan-hong ZHOU
;
Wen-ling ZHANG
;
Xiao-ling LI
;
Ke TANG
;
Wei-fang LI
;
Gui-yuan LI
Author Information
1. Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Carrier Proteins;
Green Fluorescent Proteins;
biosynthesis;
genetics;
Humans;
Molecular Sequence Data;
Nasopharyngeal Neoplasms;
metabolism;
Neoplasm Proteins;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2005;30(6):621-624
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the expression and location of coding protein of UBAP1 gene and to understand the relationship between the expression pattern of the protein and cell carcinogenesis.
METHODS:Bioinformatics was used to analyze the protein character to provide an available clue of subsequent research. The codon frame cDNA was amplified by PCR, and subcloned into enhance green fluorescence protein (EGFP) of pEGFP-C2. The recombinant plasmid was transfected into HNE1 cells. The expression of coding protein was observed by fluorescence microscopy.
RESULTS:The expressed GFP-fusion protein generated striking green fluorescence in the cytoplasm in HNE1 cells. EGFP/UBAP1 was expressed and existed mainly in the nuclear, especially accumulated on the nuclear envelope.
CONCLUSION:The expression difference in HNE1 might be related to the carcinogenesis of NPC.
