Construction of two hepatocellular carcinoma cell models for the expression of HBV X gene with different selection characteristics.
- Author:
Xing-e HE
1
;
Jian-hua LEI
;
Xu YANG
;
Wen-long WANG
;
Hong-yu LUO
;
Jun LIANG
Author Information
1. Liver Disease Research Center, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Hepatocellular;
pathology;
Cell Line, Tumor;
Eukaryotic Cells;
metabolism;
Genetic Vectors;
Humans;
Liver Neoplasms;
pathology;
Models, Biological;
Trans-Activators;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2005;30(6):636-639
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct 2 hepatocellular carcinoma (HCC) cell models for the expression of HBV X gene with different selection characteristics.
METHODS:HepG2 HCC cells were infected with eukaryotic expression vectors with HBV X gene, pCEP4-X, and pcDNA3. 1 (+)-X. Single cell clone was selected by hygromycin and neomycin. After propagating culture for certain periods, the HBV X gene expression was identified by PCR, RT-PCR, and Western blot.
RESULTS:Single HCC cell clone with HBV X gene transferred resistant to hygromycin and neomycin was selectively cultured, and the cells could be propagated for certain periods. PCR, RT-PCR, and Western blot identified the expression of HBV X gene.
CONCLUSION:Two HCC cell models for the expression of HBV X gene with different selection characteristics have been successfully constructed.
