Effects of vasoactive intestinal peptide on LPS-induced MMP-9 expression by alveolar macrophages in rats.
- Author:
Yong-ping LIU
1
;
Cha-xiang GUAN
;
Hong-bo BAI
;
Xiao-qun QIN
;
Hui-jun LIU
Author Information
1. Department of Physiology, Xiangya Medical College, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calmodulin;
metabolism;
Cells, Cultured;
Down-Regulation;
Female;
Lipopolysaccharides;
Macrophages, Alveolar;
cytology;
metabolism;
Male;
Matrix Metalloproteinase 9;
biosynthesis;
genetics;
Protein Kinase C;
metabolism;
Rats;
Rats, Wistar;
Vasoactive Intestinal Peptide;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2005;30(6):645-649
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the role of vasoactive intestinal peptide (VIP) on LPS-induced MMP-9 expression by alveolar macrophages (AM) in rats.
METHODS:LPS-induced cultured Wistar rats AMs were treated with different concentrations of VIP (10(-10) to approximately 10(-6) mol/L) for 24 h. AMs and the supernatant were collected to measure the MMP-9 expression and activity by RT-PCR and gelatin zymography, respectively. Results The MMP-9 activity and expression of LPS-induced AMs were significantly higher than those in the control group (P < 0.01). VIP (10(-9) to approximately 10(-6) mol/L) down-regulated LPS-induced MMP-9 activity and its expression. The effects were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (P < 0.01).
CONCLUSION:VIP can decrease LPS-induced MMP-9 activity and its expression, which may be related to protein kinase C and calmodulin pathway. VIP may have protective roles in the lung injury.
