Advanced oxidation protein products in diabetic nephropathy patients and its relation with superoxide dismutase, glutathione peroxidase, neopterin.
- Author:
Zhi-xian WU
1
;
Yao-ming XUE
;
Chen-zhong LI
;
Mei-ping GUAN
Author Information
1. Department of Endocrinology, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350025, China.
- Publication Type:Journal Article
- MeSH:
Aged;
Diabetes Mellitus, Type 2;
blood;
Diabetic Nephropathies;
blood;
Female;
Glutathione Peroxidase;
blood;
Humans;
Male;
Middle Aged;
Neopterin;
blood;
Oxidation-Reduction;
Oxidative Stress;
Superoxide Dismutase;
blood
- From:
Journal of Central South University(Medical Sciences)
2005;30(6):704-707
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the changes of advanced oxidation protein products (AOPP) in diabetic nephropathy (DN) patients, as well as its relationship with superoxide dismutase (SOD), glutathione peroxidase (GPx) and neopterin (NPT).
METHODS:By the concentration of urinary albumin excretion rate (UAER) and creatinine (Cr), 85 diabetes mellitus (DM) patients were divided into 4 groups as non-DN group (DM), early-staged DN group (DN3), significant DN group (DN4) and end-staged DN group (DN5). The concentration of the serum AOPP was measured by ameliorated method introduced by Wikto-Sarsat, while SOD by Xanthine oxidase test, GPx by [5,5'Dithio-bis (2-Nitrobenzoic aicd) ] (DTNB) reaction test and NPT by ELISA.
RESULTS:AOPP in Group DN5 [(117.8 +/- 64.8) [micromol/L] and Group DN4 [ (80.0 +/- 23.0) micromol/L] were significantly higher than those in Group DM [(58.2 +/- 17.7) micromol/L]. There was no significant difference of AOPP between Group DN3 [(72.7 +/-17.2) micromol/L] and Group DM. Serum AOPP negatively correlated with SOD and GPx (r = -0.217 and -0.374 respectively, P < 0.05), while positively correlated with NPT (r = 0.499, P < 0.01).
CONCLUSION:DN patient has enhanced protein oxidation than DM patient, which is related to oxidative stress and chronic inflammation in DN.
