Gene transfection efficiency and destination of recombinant adenovirus gene transfer through internal jugular vein injection in rats.
- Author:
Yu-hang AI
1
;
Li-na ZHANG
;
Hua GONG
;
Liu PENG
;
Yang-de ZHANG
Author Information
1. Intensive Care Unit, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Animals;
Female;
Gene Transfer Techniques;
Genetic Therapy;
Injections, Intravenous;
Jugular Veins;
Lac Operon;
genetics;
Lung;
metabolism;
Male;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins;
genetics;
Transfection
- From:
Journal of Central South University(Medical Sciences)
2005;30(6):653-656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the adenovirus-mediated LacZ gene expression and the destination in different organs of SD rats after the intravenous injection in rats.
METHODS:Recombinant adenovirus vector containing LacZ was transferred to SD rats by injecting into the internal jugular vein. To identify the sites and periods of LacZ gene expression, X-gal staining was used to detect beta-gal level and period of LacZ gene expression of different organs in the transfected and non-transfected rats at different time intervals.
RESULTS:On the 1st day after the injection, the lung, liver, kidney, and spleen expressed some beta-gal; on the 3rd day after the injection, the lung, liver, kidney, and spleen expressed beta-gal obviously; their peak levels were on the 7th day; the beta-gal level decreased on the 14th day; beta-gal expression disappeared in the most organs except the lungs on the 28th day. In all animals, the brain did not express any beta-gal.
CONCLUSION:The adenovirus-mediated exogenous gene transfer in the internal jugular vein may be an effective approach of gene therapy in some diseases in the lung, liver, and kidney.
