CT appearance and p16 gene abnormality of peripheral lung cancer.
- Author:
Xiu-Bin PENG
1
;
Yun-Hui LUO
;
Zhong-Li DU
;
Shi-Kui HUANG
;
Jing HE
Author Information
1. Department of Radiology, Third Affiliated Hospital of Jinan University, Zhuhai 519000, China. zhpengxiubin@sina.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
diagnostic imaging;
genetics;
Female;
Gene Deletion;
Genes, p16;
Humans;
Lung Neoplasms;
diagnostic imaging;
genetics;
Male;
Middle Aged;
Point Mutation;
Tomography, X-Ray Computed
- From:
Journal of Central South University(Medical Sciences)
2005;30(2):171-175
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between the mutation and abnormal expression of p16 gene in peripheral lung carcinoma and its CT manifestations.
METHODS:Immunohistochemistry and PCR-SSCP were used to detect P16 protein expression and p16 gene mutation of 52 cases of peripheral lung cancer. All patients were scanned with spiral CT before the operation and proved by pathology.
RESULTS:Of the 52 cases of lung cancer tissues, the negative expression rate of p16 gene protein was 53.8% (28/52), and the deletion or mutation rate of the exon 2 was 23.1% (12/52). There were significant statistical differences of p16 gene defect and its protein loss rates among groups of different clinical stages (P < 0.05), but among groups of different tissue types, different differentiation degree p16 gene defect and its protein loss rates showed no significant statistical difference (P > 0.05). In lung cancer patients with CT appearances of thin spicule, speculated protuberance, pleural indentation, and metastasis of lymph node, p16 gene and its protein loss rates were much higher than those without CT manifestations mentioned above (P < 0.05). However, there were no statistical differences among groups of different tumor sizes, with or without lobulation, with or without cavity, and different contrast enhanced CT values (P > 0.05).
CONCLUSION:p16 gene mutation and abnormal expression may play an important role in the occurrence and development of lung cancer, and it is relative to CT appearances of lung cancer. p16 gene may be used as a predicting index for clinical diagnosis and prognosis assessment.
