Immunogenicity of bovine jugular vein conduits treated with different cross-linking methods.
- Author:
Hui WANG
1
;
Jian-guo HU
;
Zhong-shi WU
;
Tei-hui HU
;
You-hua DENG
;
Yao-guang FENG
;
Zhi-bing JIANG
;
Yan-qing YANG
Author Information
1. Department of Cardiothoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bioprosthesis;
Blood Vessel Prosthesis;
Cattle;
Cross-Linking Reagents;
Glutaral;
pharmacology;
Jugular Veins;
immunology;
Prostheses and Implants;
Rabbits;
Random Allocation
- From:
Journal of Central South University(Medical Sciences)
2005;30(2):176-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the immunogenicity of bovine jugular vein conduits (BJVCs) treated with different cross-linking methods.
METHODS:The BJVCs were treated with glutaradehyde (GA), dye-mediated photooxidation (DMP) and polyepoxy compound (PC) (n = 10). The tissue homogenates obtained from BJVCs treated with PC, GA, DMP, and fresh BJVCs, were mixed with the complete Freand adjavant to form the emulsive antigen, which were used to immunize rabbits correspondently. The antibody concentrations to BJVCs in those rabbits' serum were measured by counter double immuno diffusion. The immunologic responses to the BJVCs in different groups were measured with Western blotting.
RESULTS:The positive bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with antigens of fresh BJVCs, but no bands appeared when the sera of rabbits were immunized by fresh BJVCs reacted with those antigens of the BJVCs treated with GA, DMP, and PC in Western blotting.
CONCLUSION:The immunogenicity of BJVCs treated with PC, DMP, and GA can be reduced significantly and meet the clinical standard.
