Effects of CD147 on the production of matrix metalloproteinase-9 by fibroblasts and the invasion of melanoma cells.
- Author:
Xiang CHEN
1
;
Li-Hong TIAN
;
Hong-Fu XIE
;
Wei SHI
;
Hao FENG
;
Ji LI
;
Fu-Wen CHEN
Author Information
1. Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008. China. chenxck@yahoo.com
- Publication Type:Journal Article
- MeSH:
Basigin;
immunology;
pharmacology;
Coculture Techniques;
Fibroblasts;
cytology;
metabolism;
Humans;
Matrix Metalloproteinase 9;
biosynthesis;
genetics;
Melanoma;
pathology;
Neoplasm Invasiveness;
Tumor Cells, Cultured
- From:
Journal of Central South University(Medical Sciences)
2005;30(3):249-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate a possible role of CD147 in the production of matrix metalloproteinase-9 (MMP-9) by fibroblasts and the invasion of melanoma cells.
METHODS:We cocultured CD147-expression melanoma cells with fibroblasts and examined the MMP-9 expression of fibroblasts by zymography and the invasion of melanoma cells by transwell invasion assay.
RESULTS:MMP-9 expression was enhanced in conditioned media, while cocultured with melanoma cells in a dose-dependent manner, and CD147 antibody inhibited the production of MMP-9 in the fibroblasts. When fibroblasts were cultured at the bottom of the lower compartment of transwell invasion model, the number of melanoma cells that invaded significantly increased. Addition of anti-CD147 antibody to the upper compartment transwell invasion model resulted in the significant inhibition of the melanoma cell invasion in reconstituted basement membrane.
CONCLUSION:CD147 expressed in melanoma cells plays an important role in the melanoma cell invasion by stimulating the production of MMP-9 by fibroblasts.
