Differentiation of mesenchymal stem cells into cardiomyocytes induced by cardiomyocytes.
- Author:
Ting-Zhong WANG
1
;
Ai-Qun MA
;
Zheng-Yun XU
;
Wen-Hui JIANG
;
Yuan DU
Author Information
1. Department of Cardiovascular Medicine, First Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD34;
analysis;
Bone Marrow Cells;
cytology;
Cell Communication;
Cell Differentiation;
physiology;
Cell Separation;
Cells, Cultured;
Coculture Techniques;
Female;
Male;
Mesenchymal Stem Cells;
cytology;
Myocytes, Cardiac;
cytology;
Rats;
Rats, Sprague-Dawley;
Thy-1 Antigens;
analysis
- From:
Journal of Central South University(Medical Sciences)
2005;30(3):270-275
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of adult cardiomyocytes in the differentiation of mesenchymal stem cells (MSCs) into cardiomyocytes.
METHODS:Rat MSCs were isolated by a Percoll's gradient solution and cultured in low-glucose Dulbecco' s modified Eagle' s medium (DMEM). After 2 passages, cell-surface antigen CD34, CD71 and CD90 for rat MSCs were determined by flow cytometry, and these MSCs were transfected with pEGFP-N3 by Lipofectamine2000. Then those MSCs labeled with GFP, were cultured in contacted, nocontacted and conditioned with adult rat myocardiocytes. Immunofluorescence staining against alpha-actin, desmin, and troponin-T were performed after 1 week.
RESULTS:Immunofluorescence staining was positive against alpha-actin, desmin, and troponin-T on MSCs in contacted culture group. In contrast, no alpha-actin, desmin, and troponin-T expression on MSCs were observed in the noncontacted culture group and the conditioned culture group.
CONCLUSION:Direct cell-to-cell contact between MSCs and adult cardiomyocytes may induce differentiation of MSCs into cardiomyocytes.
