Effects of astragalus polysaccharides-chitosan/polylactic acid composite material on biological behavior of canine bone marrow stromal cells cultured in vitro.
- Author:
Chun-Jiao XU
1
,
2
;
Xin-Chun JIAN
;
Jie-Ying PENG
;
Feng GUO
;
Bai-Ying HUANG
;
Cheng-Dong XIONG
;
Gao-Feng PAN
Author Information
1. Department of Periodontology
2. Xiangya Hospital, Central South University, Changsha, China. jianxinchun@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Astragalus propinquus;
Bone Marrow Cells;
cytology;
Cell Proliferation;
Cells, Cultured;
Chitosan;
pharmacology;
Collagen Type I;
biosynthesis;
Dogs;
Drugs, Chinese Herbal;
pharmacology;
Extracellular Matrix;
metabolism;
Female;
Lactic Acid;
pharmacology;
Male;
Osteocalcin;
biosynthesis;
Polyesters;
Polymers;
pharmacology;
Polysaccharides;
pharmacology;
Stromal Cells;
cytology;
Tissue Engineering
- From:
Journal of Central South University(Medical Sciences)
2005;30(3):283-287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the biological behavior of canine bone marrow stromal cells (BMSCs) cultured in vitro with the astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) and with the chitosan/polylactic acid (C/PLA) and to find a suitable compound material for periodontal tissue engineering.
METHODS:BMSCs (induced 14 days by 50 mg/L vitamine C, 10(-8) mol/L dexamethasone, 10 mmol/L beta-sodium glycerylphosphate) were cultured on AP-C/PLA or C/PLA for 5 days respectively. The BMSCs attachment and the morphology were observed with scanning electronic microscope and the combining rates were counted. Type I collagen synthesis was examined with immunohistochemistry staining and the content of osteocalin was determined with radio-immunological method.
RESULTS:Combining rates, type I collagen synthesis, and the content of osteocalin of BMSCs on AP-C/PLA were significantly higher than those on C/PLA.
CONCLUSION:AP-C/PLA may promote the BMSC proliferation, differentiation and extracellular matrix synthesis, and it can be used as a good scaffold material for bone tissue engineering.
