Differentiation-inducing effects of perphenazine on K562 leukemia cells.
- Author:
Lin-Bo YUAN
1
;
Qun HE
Author Information
1. Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Cell Transformation, Neoplastic;
drug effects;
Flow Cytometry;
Humans;
K562 Cells;
Perphenazine;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2005;30(3):288-291
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the differentiation-inducing effects of perphenazine on K562 leukemia cells.
METHODS:Differentiation-Inducing effects of a phenothiazine perphenazine were evaluated by proliferation, morphology and function of K562 cells. We evaluated the effects of perphenazine on K562 cells proliferation by cellular enumeration in liquid culture assay, MTT assay and clony formation assay, the morphology by Wight-Gimesa staining, and the function by detecting CD71 through flow cytometry.
RESULTS:Perphenazine enhanced the expression of CD71 on K562 cells and increased Hb content in K562 cells, while inhibited the proliferation of K562 cells. K562 cells showed differentiation morphology after the drug treatment.
CONCLUSION:Perphenazine possessed differentiation-inducing effects on K562 cells.
