Role of spinal P2X4 receptor in remifentanil-induced postoperative hyperalgesia.
10.11817/j.issn.1672-7347.2019.04.005
- Author:
Wenxiang QING
1
;
Jianqin YAN
2
;
Chengliang ZHANG
3
;
Junjie ZHANG
2
;
Zhenping ZHAI
2
;
Jiajia HU
2
Author Information
1. Department of Anesthesiology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
2. Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
3. Department of Cardiovascular Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Hyperalgesia;
Male;
Pain, Postoperative;
Rats;
Rats, Sprague-Dawley;
Receptors, Purinergic P2X4;
Remifentanil;
Spinal Cord
- From:
Journal of Central South University(Medical Sciences)
2019;44(4):370-376
- CountryChina
- Language:Chinese
-
Abstract:
To explore the role of P2X4 receptor in opioid-induced hyperalgesia (OIH).
Methods: A total of 30 Sprague-Dawley (SD) male rats were randomly divided into 5 groups: a saline (N0) group, a remifentanil at 0.5 μg/(kg.min) (R1) group, a remifentanil at 1.0 μg/(kg.min) (R2) group, a remifentanil at 1.5 μg/(kg.min) (R3) group, and a remifentanil at 5.0 μg/(kg.min) (R4) group. The paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured at follow time points to optimize the dosages: the day before treatment (T1), 30 min after tail intravenous catheterization (T2), and 30 min (T3), 1 h (T4), 2 h (T5), 24 h (T6) after withdrawal from remifentanil. Then, the rats were randomly divided into 2 groups: a saline group (N group), a remifentanil at 1.0 μg/(kg.min) group (R group). The PWMT and PWTL were measured at follow time points: T1, T2, and T4. The lumbar enlargement of spine was selected at 1 h after withdrawal from remifentanil, and the expression of P2X4 receptor mRNA and protein was examined in OIH. Additional male rats were selected and randomly divided into 2 groups: a plantar incision surgery followed by saline treatment group (I+N group), a plantar incision surgery followed by remifentanil treatment group (I+R group). The PWMT and PWTL were measured at follow time points: T1, T2, T3, T4, T5, T6, 48 h (T7) and 72 h (T8) after withdrawal from remifentanil. The lumbar enlargement of spine was selected at 1 h after withdrawal from remifentanil, the expression of P2X4 receptor mRNA and protein was examined by PCR and Western blotting, and the microglial activation in spine 1 h after withdrawal from remifentanil were assessed by immunofluorescence.
Results: The pain thresholds including PWMT and PWTL in different groups were as follows: R4 group
