Effects of chrysin on the apoptosis in oral squamous carcinoma KB cell line and the underlying mechanisms.
10.11817/j.issn.1672-7347.2019.05.008
- Author:
Yaxin XIE
1
;
Xiaoning PENG
2
Author Information
1. West China College of Stomatology, Sichuan University, Chengdu 610041, China.
2. Jishou University School of Medicine, Jishou Hunan 416000, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Carcinoma, Squamous Cell;
Flavonoids;
Humans;
KB Cells;
Mouth Neoplasms;
Phosphatidylinositol 3-Kinases;
Signal Transduction
- From:
Journal of Central South University(Medical Sciences)
2019;44(5):522-527
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of chrysin on apoptosis of oral squamous carcinoma KB cell line and the possible mechanisms, and to provide new ideas for the treatment of oral cancer.
Methods: Oral cancer KB cells were treated with different concentrations of chrysin (1, 2, 4, 8, 16, and 32 μmol/L) for 24 h. Cell proliferation was detected by MMT assay; apoptosis was detected by flow cytometry; the activity of caspase-3/7 was detected by chemiluminescent assay; mitochondrial membrane potential in KB cells was determined by JC-1 assay; and Western blotting was used to determine the activation of protein kinase B (AKT) and phosphoinositide-3-kinase (PI3K).
Results: Chrysin inhibited the proliferation of KB cells in a concentration-dependent manner, accompanied by increase in apoptosis of KB cells, activation of caspase-3/7, decrease in mitochondrial membrane potential, and suppression of the phosphorylation of AKT and PI3K.
Conclusion: The effect of chrysin on KB cell apoptosis may be related to mitochondrial dysfunction and inhibition of PI3K/AKT pathway.
