Regulation of osteogenic differentiation of human adipose-derived stem cells by controlling electromagnetic field conditions.
- Author:
Kyung Shin KANG
1
;
Jung Min HONG
;
Jo A KANG
;
Jong Won RHIE
;
Young Hun JEONG
;
Dong Woo CHO
Author Information
1. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea. dwcho@postech.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
adipose-derived stem cells;
electromagnetic field;
frequency;
magnetic flux density;
optimization;
osteogenic differentiation
- MeSH:
Adipose Tissue/*cytology;
Alkaline Phosphatase/metabolism;
Biological Markers/metabolism;
Bone Matrix/metabolism;
Calcification, Physiologic/genetics;
*Cell Differentiation/genetics;
Core Binding Factor Alpha 1 Subunit/metabolism;
*Electromagnetic Fields;
Humans;
*Osteogenesis/genetics;
Reproducibility of Results;
Stem Cells/*cytology/enzymology/metabolism
- From:Experimental & Molecular Medicine
2013;45(1):e6-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Many studies have reported that an electromagnetic field can promote osteogenic differentiation of mesenchymal stem cells. However, experimental results have differed depending on the experimental and environmental conditions. Optimization of electromagnetic field conditions in a single, identified system can compensate for these differences. Here we demonstrated that specific electromagnetic field conditions (that is, frequency and magnetic flux density) significantly regulate osteogenic differentiation of adipose-derived stem cells (ASCs) in vitro. Before inducing osteogenic differentiation, we determined ASC stemness and confirmed that the electromagnetic field was uniform at the solenoid coil center. Then, we selected positive (30/45 Hz, 1 mT) and negative (7.5 Hz, 1 mT) osteogenic differentiation conditions by quantifying alkaline phosphate (ALP) mRNA expression. Osteogenic marker (for example, runt-related transcription factor 2) expression was higher in the 30/45 Hz condition and lower in the 7.5 Hz condition as compared with the nonstimulated group. Both positive and negative regulation of ALP activity and mineralized nodule formation supported these responses. Our data indicate that the effects of the electromagnetic fields on osteogenic differentiation differ depending on the electromagnetic field conditions. This study provides a framework for future work on controlling stem cell differentiation.
