MicroRNA-21 correlates TGF-β1 pathway of pancreatic ductal adenocarcinoma.
10.11817/j.issn.1672-7347.2019.180708
- Author:
Xiang WU
1
;
Shuwei ZHUO
1
;
Cailing ZHENG
1
;
Ge GAO
2
Author Information
1. Clinical Laboratory, Hainan Provincial Hospital of Chinese Medicine, Haikou 570203, China.
2. Department of Laboratory Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Pancreatic Ductal;
genetics;
Humans;
MicroRNAs;
genetics;
Pancreatic Neoplasms;
genetics;
Transforming Growth Factor beta1
- From:
Journal of Central South University(Medical Sciences)
2019;44(7):749-756
- CountryChina
- Language:Chinese
-
Abstract:
To conduct genetic analysis of pancreatic ductal adenocarcinoma tissues and analyze the correlation between targeted microRNA (miRNA) and pathways in pancreatic ductal adenocarcinoma.
Methods: We collected 19 samples of peripheral venous blood serum from patients with pancreatic ductal adenocarcinoma in Hainan Provincial Hospital of Chinese Medicine, and also collected 21 blood serum samples as a control group of non-pancreatic ductal adenocarcinoma. We used the bioinformatics analysis of literature GCBI data platform for screening and analyzing the genetics of pancreatic ductal adenocarcinoma samples. Through GCBI data platform of hierarchy clustering analysis and the enrichment of gene function analysis, the relevant miRNA was screened as a research object in patients with pancreatic ductal adenocarcinoma. The miRNA was screened by literature analysis and pancreatic cancer gene analysis. Real-time PCR and Western blotting were carried out to study the relationship between the selected miRNA and TGF-β1 by overexpression and suppression of the gene in pancreatic ductal adenocarcinoma cells.
Results: MiRNA-21 was screened as a gene associated with pancreatic ductal carcinoma via hierarchy clustering analysis and gene function analysis. MiRNA-21 was highly expressed in the pancreatic ductal carcinoma patients. Expressions of TGF-β1 were inhibired in miRNA-21 overexpressed PANC-1. While the expression of miRNA-21 was inhibited, TGF-β1 expression increased obviously.
Conclusion: MiRNA-21 is highly expressed in patients with pancreatic ductal adenocarcinoma, can regulate the expression of TGF-β1, which may be a mechanism of miRNA-21 in pancreatic ductal adenocarcinoma.
