Inhibitory effect of andrographolide on angiogenesis induced by the supernatant from cultured tumor cells.
10.11817/j.issn.1672-7347.2018.08.001
- Author:
Xiaolan GUO
1
;
Maozhou ZHAO
1
;
Yuyin LIN
1
;
Wensheng CHEN
1
;
Shiwen WANG
1
;
Jianwei DAI
1
Author Information
1. GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou 511436, China.
- Publication Type:Journal Article
- MeSH:
Capillaries;
drug effects;
Cell Survival;
Collagen;
Culture Media;
Diterpenes;
pharmacology;
Drug Combinations;
Human Umbilical Vein Endothelial Cells;
drug effects;
Humans;
Laminin;
Matrix Metalloproteinase 9;
metabolism;
Neovascularization, Pathologic;
enzymology;
etiology;
prevention & control;
Proteoglycans;
Tumor Cells, Cultured
- From:
Journal of Central South University(Medical Sciences)
2018;43(8):821-825
- CountryChina
- Language:Chinese
-
Abstract:
To determine the effect of andrographolide (Andro) on angiogenesis of human umbilical vein endothelial cells (HUVECs).
Methods: HUVECs were treated with different concentrations of Andro and the cell viability was detected with Cell Counting Kit-8 (CCK-8). HUVECs were treated with half lethal dose (IC50) of Andro. Matrigel was used to make capillary formation of HUVECs and the effect of Andro on capillary formation was evaluated by calculating the percentage of capillary formation. Moreover, the effects of Andro and the supernatant from cultured A549 tumor cells on capillary formation were evaluated by calculating the percentage of capillary formation. The effect of Andro on the expression of matrix metalloproteinase-9 (MMP-9) was determined with Western blot.
Results: The cell viability of HUVECs decreased with the increase of Andro concentrations. IC50 was 20 μmol/L. The capillary formation of HUVECs was inhibited when treated with 20 μmol/L Andro for 24 hours. Moreover, Andro was able to antagonize the promotion of the capillary formation induced by the supernatant from cultured tumor cells. Andro could suppress the expression of MMP-9 and antagonize the capillary formation.
Conclusion: Andro inhibits the capillary formation of HUVECs and can antagonize the promotion of angiogenesis induced by the supernatant from cultured tumor cells.
