Effect of EGCG on oxidative stress and Nrf2/HO-1 pathway in neurons exposed to oxygen-glucose deprivation/reperfusion.
10.11817/j.issn.1672-7347.2018.10.001
- Author:
Fang HE
1
;
Yi ZHANG
2
;
Shang CHEN
2
;
Bei YE
2
;
Jianzhen CHEN
2
;
Chang LI
3
Author Information
1. Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu 215009, China fanghe126@163.com.
2. Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu 215009, China.
3. Department of Thoracic Surgery, First Affiliated Hospital of Soochow University, Suzhou Jiangsu 215006, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Catechin;
analogs & derivatives;
pharmacology;
Cell Survival;
drug effects;
Cells, Cultured;
Gene Expression Regulation;
drug effects;
Glucose;
Heme Oxygenase-1;
genetics;
metabolism;
NF-E2-Related Factor 2;
genetics;
metabolism;
Neurons;
drug effects;
Neuroprotective Agents;
pharmacology;
Oxidative Stress;
drug effects;
Oxygen;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury;
prevention & control
- From:
Journal of Central South University(Medical Sciences)
2018;43(10):1041-1047
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of epigallocatechin gallate (EGCG) on oxidative stress and Nrf2/HO-1 pathway in neurons subjected to oxygen-glucose deprivation/reperfusion (OGD/R).
Methods: Primary cultured cerebral cortical neurons were prepared from Sprague-Dawley rats, and the OGD/R cell model was established. After pretreatment with EGCG at different concentrations (12.5, 25.0, 50.0 or 100.0 μmol/L), the neurons were subjected to OGD/R. The cell viability, reactive oxygen species (ROS) level and malondialdehyde (MDA) content were assessed after reperfusion. The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were measured. The expression of Nrf2 protein in nucleus, HO-1 mRNA and protein were detected.
Results: OGD/R treatment reduced the cell viability, elevated ROS level and MDA content, decreased SOD and GSH-Px activities. The expression of Nrf2 protein in nucleus, HO-1 mRNA and protein were increased (P<0.01). Pretreatment with EGCG promoted the survival of neurons exposed to OGD/R, decreased ROS level and MDA content while increased SOD and GSH-Px activities. The levels of Nrf2 protein in nucleus, HO-1 mRNA and protein were upregulated (P<0.01).
Conclusion: EGCG can reduce the oxidative stress of neurons subjected to OGD/R, which may be related to activation of Nrf2/HO-1 signal pathway and enhancement of the antioxidant ability of neurons.
