Effect of ursolic acid on invasion and migration of hepatocellular carcinoma cells co-cultured with macrophages and the underlying mechanisms.
10.11817/j.issn.1672-7347.2018.11.004
- Author:
Yaoyao LI
1
;
Enhua SHEN
2
Author Information
1. Medical College, Jingchu University of Technology, Jingmen Hubei 448000, China ql9872@163.com.
2. Department of Infection, Fourth Hospital of Jilin University, Changchun 130000, China.
- Publication Type:Journal Article
- MeSH:
Cadherins;
genetics;
Carcinoma, Hepatocellular;
pathology;
Cell Line, Tumor;
Cell Movement;
drug effects;
Coculture Techniques;
Epithelial-Mesenchymal Transition;
Gene Expression Regulation, Neoplastic;
drug effects;
Humans;
Liver Neoplasms;
pathology;
Macrophages;
cytology;
Neoplasm Invasiveness;
pathology;
Triterpenes;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2018;43(11):1188-1193
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of ursolic acid on the invasion and migration of hepatocellular carcinoma (HCC) cells co-cultured with macrophages, and to explore the underlying mechanisms.
Methods: The migration and invasion ability of HCC cells in the co-culture system with or without ursolic acid intervention were evaluated by transwell assay. The levels of epithelial-mesenchymal transition (EMT) markers E-cadherin, N-cadherin, and vimentin in HCC cells co-cultured with macrophages were detected by Western blot.
Results: The migration and invasion ability and EMT were significantly enhanced when co-cultured with macrophages, and the expression of E-cadherin was significantly increased while N-cadherin and vimentin levels were significantly decreased. However, after ursolic acid treatment, the migration and invasion ability were significantly reduced, and the expression of E-cadherin was increased while N-cadherin and vimentin levels were decreased.
Conclusion: Ursolic acid exerts inhibitory effect on the ability of migration, invasion, and EMT for HCC, which are enhanced by co-culturing with macrophages.
