Aucubin alleviates lipopolysaccharide-induced acute lung injury in mice.
10.11817/j.issn.1672-7347.2019.02.003
- Author:
Bingbing PAN
1
;
Yong ZHOU
2
;
Gaoyin KONG
3
;
Ping LI
2
;
Qulian GUO
1
;
Chaxiang GUAN
2
Author Information
1. Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
2. Department of Physiology, School of Basic Medicine Science, Central South University, Changsha 410078, China.
3. Department of Anesthesiology, Hunan Provincial People's Hospital, Changsha 410005, China.
- Publication Type:Journal Article
- MeSH:
Acute Lung Injury;
chemically induced;
Animals;
Bronchoalveolar Lavage Fluid;
Iridoid Glucosides;
Lipopolysaccharides;
Lung;
Male;
Mice;
Tumor Necrosis Factor-alpha
- From:
Journal of Central South University(Medical Sciences)
2019;44(2):128-133
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of prophylactic aucubin (AU) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.
Methods: Male BABL/c mice were randomly divided into a control group, an ALI group, and an AU treatment group, 16 mice in each group. ALI mice were injected with LPS (5 mg/kg, intratracheal injection), and AU (10 mg/kg) was injected intraperitoneally 30 min ahead. After LPS injection for 6 hours mice were sacrificed, the morphological changes of lung tissues were detected by HE staining and the lung injury score was obtained. The mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin 10 (IL-10) in lung tissue was detected by real-time PCR. The total protein and lactate dehydrogenase (LDH) activity, the cell count, and the protein content of TNF-α and IL-10 in the mouse bronchoalveolar lavage fluid (BALF) were detected.
Results: Compared with ALI mice, the pathological damage score of lung tissue was significantly reduced in the AU group, the total number of BALF cells, neutrophils, and macrophages were significantly decreased, LDH activity and the total protein content were also significantly decreased (all P<0.01). In addition, AU can reduce the mRNA and protein expression of TNF-α in lung of ALI mice, and increase the mRNA and protein expression of IL-10 (all P<0.01).
Conclusion: AU can reduce LPS-induced ALI in mice.
