Icaritin prevents vascular calcification in mice.
10.11817/j.issn.1672-7347.2019.01.006
- Author:
Lu REN
1
;
Zun WANG
2
;
Qingzhong HUA
2
;
Hui XIE
3
;
Siyuan TANG
2
Author Information
1. Department of Clinical Nursing Teaching and Research Section, Second Xiangya Hospital, Central South University, Changsha 410011, China.
2. Xiangya Nursing School, Central South University, Changsha 410013, China.
3. Department of Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Aorta;
Flavonoids;
Male;
Mice;
Mice, Inbred C57BL;
Muscle, Smooth, Vascular;
Vascular Calcification;
prevention & control
- From:
Journal of Central South University(Medical Sciences)
2019;44(1):35-39
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of icaritin on preventing the vascular calcification in mouse induced by vitamin D2.
Methods: Fifty male C57BL mice were randomly assigned to a control group (n=10) and a model group (n=40). Mice in model group were treated with gradient-concentration of icaritin for 8 weeks. Then, they were consecutively treated with vitamin D2 for 4 days. Meanwhile, mice in negative control group were treated with the same dosage of PBS. At the end of the treatment, aortae were collected to examine the concentration of Ca, the area of calcification and the expression of α-smooth muscle actin (α-SMA) and Runx2.
Results: Compared with the negative control group, the weight of mice and the concentration of Ca in the positive control (vitamin D2+0 mg/kg icaritin) group declined significantly (P<0.05) after injection of vitamin D2. Compared with the positive control group, the Ca concentration, the area of calcification, and the expression of Runx2 were significantly declined at the middle dosage of icaritin (vitamin D2+0.2 mg/kg icaritin) group (P<0.05).
Conclusion: Icaritin could effectively prevent the vascular calcification in mice.
