P38 MAPK inhibitor SB203580 attenuates the toxicity of ropivacaine on PC12 cells.
10.11817/j.issn.1672-7347.2019.180602
- Author:
Yuan CHEN
1
;
E WANG
1
;
Zhihua SUN
2
;
Zongbin SONG
1
;
Zhi YE
1
;
Zhong ZHANG
1
Author Information
1. Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
2. Department of Anesthesiology, Xiangya Changde Hospital, Changde Hunan 415000, China.
- Publication Type:Journal Article
- MeSH:
Anesthetics, Local;
toxicity;
Animals;
Apoptosis;
Imidazoles;
PC12 Cells;
Pyridines;
Rats;
Ropivacaine;
toxicity;
p38 Mitogen-Activated Protein Kinases;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2019;44(9):985-989
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of SB203580, a p38MAPK specific inhibitor, on ropivacaine-induced cytotoxicity in PC12 cells.
Methods: PC12 cells were divided into three groups: the normal group (Group N), cells were cultured for 48 h; the ropivacaine group (Group R), cells were cultured with 15 mmol/L ropivacaine hydrochloride for 48 h; the ropivacaine+SB203580 group (Group R+S), cells were cultured with 15 mmol/L ropivacaine hydrochloride plus 10 μmol/L SB203580 for 48 h. The cell survival rates were detected by MTT assay. The protein levels of cleaved caspase-3, phosphor-p38 (p-p38) and cystolic cytochrome C (Cyt C) were detected by Western blotting.
Results: Compared with the Group N, the number and survival rate of PC12 cells in the Group R and the Group R+S were significantly reduced (all P<0.05); the number and survival rate of PC12 cells in the Group R+S were significantly higher than those in the Group R (both P<0.05). Compared with the Group N, the levels of p-p38 and cleaved caspase-3, and the content of cytoplasmic Cyt C in the PC12 cells from the Group R and the Group R+S were significantly enhanced (all P<0.05); compared with the Group R, the levels of p-p38 and cleaved caspase-3, and the content of cytoplasmic Cyt C in the PC12 cells from the Group R+S were decreased (all P<0.05).
Conclusion: The ropivacaine-induced cytotoxicity can be attenuated via inhibition of p38MAPK; which is related to decrease in Cyt C content and cleaved caspase-3 expression.
